Quantitative analysis of hDectin-1 mRNA expression in different cell populations. The expression of hDectin-1 was analyzed in different in vitro–generated cell populations. Quantitative real-time RT-PCR was performed using primers discriminating the 4 isoforms hDectin-1a (■), hDectin-1b (), hDectin-1c (), and hDectin-1d (□). Expression is shown in peripheral blood monocytes differentiating into immature MDCs over a 7-day period without TLR agonist (A) and in MDCs that were matured with different TLR ligands (B). Shown are the relative expression levels in comparison with the endogenous control GAPDH. 7d or GM/IL-4 indicate immature MDCs on day 7. The data shown are representative of 3 independent experiments.