![Figure 4. Summary of depletion efficacy in 9 consecutive photodepletion (PD) experiments. Displayed P values were obtained using a 2-tailed Wilcoxon matched pair test. (A) Scatterplots (horizontal bars represent the median) of depletion efficacy in 6 mismatched experiments (Exp's I-III, VII-IX) as determined by mixed lymphocyte reactions (MLRs) one day after PD and calculated versus baseline alloreactivity of the untreated primary coculture: Depletion index = [alloreactivity of untreated control]/[alloreactivity of PD product]. (B) Scatterplots (horizontal bars represent the median) of depletion efficacy in 6 mismatched experiments (Exp's I-III, VII-IX) as determined by MLR and calculated versus baseline alloreactivity of unmanipulated responder PBMCs: Depletion index = [alloreactivity of unmanipulated responder]/[alloreactivity of PD product]. (C) Mismatched experiments (Exps I-III, VII-IX) and alloreactivity of MLRs before and after PD (N = 6). (D-F) Matched experiments (Exps IV-VI) and helper T-lymphocyte precursor (HTLp) frequency before and after PD (N = 3). Stim indicates irradiated stimulator cells; Pat, patient PBMCs; Resp, unmanipulated responder PBMCs; 3rdP, pooled third-party PBMCs; UC, untreated control; ExLy, expanded lymphocytes; PD/5.0TH and PD/7.5TH, PD products generated using 5.0 and 7.5 μM TH9402, respectively; DI, depletion index; and cpm, counts per minute.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/111/8/10.1182_blood-2007-08-104471/6/zh80020811370004.jpeg?Expires=1722438174&Signature=Sjh1Bfu0qEcI6OUIF0uxuw7v3wO5dbL1dtEf4uhJnTNy1k82nMyf-EWBM~B-0p1FkZTHCqI4d7~k2snkd1XN90EVX~KkDon6JAlNFmRNv-AKDXzAqfi3STTLxiyBartQUGdLNFWbmuC6zYW8tqTnec0vbxp1AK~lt9n5EGWuNBWQhxM3-Z2qfibGY59GrNRf0-nPBE3lqe~c4HSLIJUTnUrVCQvm89WNsRhUuzsj~ZkrWFFHgcdSpSUqP2x5~6Z6kvKcmCGHgTgBNFiSEYmu0d55eNWDkgEZuSKQi2OLmecufp0qH3QNwiOeFwfeysDjoFLmFln~1jcegJshZkdBqg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Summary of depletion efficacy in 9 consecutive photodepletion (PD) experiments. Displayed P values were obtained using a 2-tailed Wilcoxon matched pair test. (A) Scatterplots (horizontal bars represent the median) of depletion efficacy in 6 mismatched experiments (Exp's I-III, VII-IX) as determined by mixed lymphocyte reactions (MLRs) one day after PD and calculated versus baseline alloreactivity of the untreated primary coculture: Depletion index = [alloreactivity of untreated control]/[alloreactivity of PD product]. (B) Scatterplots (horizontal bars represent the median) of depletion efficacy in 6 mismatched experiments (Exp's I-III, VII-IX) as determined by MLR and calculated versus baseline alloreactivity of unmanipulated responder PBMCs: Depletion index = [alloreactivity of unmanipulated responder]/[alloreactivity of PD product]. (C) Mismatched experiments (Exps I-III, VII-IX) and alloreactivity of MLRs before and after PD (N = 6). (D-F) Matched experiments (Exps IV-VI) and helper T-lymphocyte precursor (HTLp) frequency before and after PD (N = 3). Stim indicates irradiated stimulator cells; Pat, patient PBMCs; Resp, unmanipulated responder PBMCs; 3rdP, pooled third-party PBMCs; UC, untreated control; ExLy, expanded lymphocytes; PD/5.0TH and PD/7.5TH, PD products generated using 5.0 and 7.5 μM TH9402, respectively; DI, depletion index; and cpm, counts per minute.
