DDX3Y peptide–specific T-cell responses in a F→M hematopoietic cell transplant recipient experiencing GVL. PBMCs obtained from UPN 21234 (HLA-A*01, -A*02, -B*07, -B*15, -DRB1*0401/0404, and -DQB1*03) on day +127 after transplantation were stimulated in vitro with a pool of 36 overlapping DDX3Y-derived pentadecapeptides pulsed onto donor-derived EBV-LCLs and subsequently analyzed by IFN-γ ELISpot for DDX3Y peptide–specific responses (Tables 1, 2). (A) ELISpot analysis of day +127 PBMCs that had been stimulated in vitro with the entire pool of 36 DDX3Y-derived pentadecapeptides. Asterisks indicate the 4 peptide pools that stimulated spot formation above background. (B) An aliquot of the T-cell line analyzed in panel A was restimulated with pentadecapeptide no. 17, and subsequently analyzed by ELISpot for reactivity with the SRDSRGKPGY decamer, encompassed within the sequence of pentadecapeptide no. 17.