CTL 68H7-819 targets the leukemic stem cell in AML. Flow cytometric analysis using human PE-conjugated antihuman CD45 and FITC-conjugated anti–HLA-B27 antibodies of BMMCs from representative mice injected with PBS (sham), or with AML cells that had either been cultured overnight in medium alone, with CTL 68H7-819, or with an irrelevant CD8⁺ CTL clone that did not recognize the AML in vitro. Top panels show human Y-chromosome–specific PCR analysis of genomic DNA isolated from BMMCs (BM), peripheral blood (PB), spleen (Sp), thymus (Thy), lung (Lg), liver (Lv), and kidney (Kid). The flow cytometry and PCR data from 2 representative mice from each group are shown, and are designated as A or B.