Reconstitution with wild-type bone marrow was sufficient to clear excess ligand. Serum levels of CX3CL1 (A) and CXCL1 (B) were measured by ELISA before and 6 weeks after bone marrow transfer. The results reveal that reconstitution with wild-type cells significantly decreased serum levels of ligands to levels comparable with wild-type mice. Similarly, measurement of chemokines in CNS tissue of mice shows that CX3CL1 (C) and CXCL1 (D) were reduced 6 weeks after reconstitution with wild-type bone marrow. Clearance of CX3CL1 by CX3CR1 was evaluated in an in vitro brain slice preparation (E). Bars show mean value with 95% CI of CX3CL1 in pg/mL. Each point represents a value from individual wells in 2 independent experiments.