Etsrp affects both hematopoiesis and vasculogenesis in the anterior but not the posterior region. (A-D) Posterior ectopic pu.1 expression is independent of etsrp function as analyzed by in situ hybridization at 22 hpf. (A) Control uninjected embryo; (B) embryo injected with etsrp MOs; (C) gata1 MO-injected embryo; (D) gata1 MO and etsrp MO coinjected embryo. Note that ectopic pu.1 expressed in the erythroid cells in gata1 morphants (C) is unaffected in the double gata1/etsrp morphants (D). Anterior myeloid-specific pu.1 expression (, A,C) is missing in etsrp morphants (B,D). (E-H) Anterior ectopic gata1 expression is dependent on etsrp function as analyzed by the in situ hybridization at 20 hpf to 21 hpf. (E) Control uninjected embryo; (F) embryo injected with etsrp MOs; (G) pu.1 MO-injected embryo; (H) etsrp and pu.1 MOs coinjected embryo. Note that the ectopic myeloid-specific anterior gata1 expression in pu.1 morphants (, G) is absent in double etsrp/pu.1 morphants (H). Posterior erythroid gata1 expression is not affected in etsrp morphants. Images were taken using Axioskop2 and 5×/0.15 NA dry objective, Axiocam color camera (Zeiss, model 412-312) and Openlab 4.0 software (Improvision). Magnification: 75×.