Localization of microtubule plus ends in the platelet marginal band. (A) Methanol-fixed resting mouse platelets labeled with anti-EB1 antibodies. EB1 “comets” () are labeled along the marginal band. An average of 8.66 (± 2.11; n = 26) comets were observed along the marginal band. (B) Histogram showing EB1 signals observed along the microtubule coil of each mouse platelet. The number of signals ranged from 4 to 12, with a median of 8. (C) Anti-EB1 staining in resting human platelets. EB1 comets () are identified. An average of 8.9 (± 1.5; n = 34) comets are seen along each coil. (D) Histogram of EB1 signals along the microtubule coil of human platelets. The number of signals ranged from 6 to 13, with a median of 9.