Figure 1
![Figure 1. Transcriptional profile of endothelial and smooth muscle markers in hMSCs. (A) RT-PCR analysis of endothelial markers (VE-cadherin and PECAM1) in hMSCs at baseline and in hMSCs cocultured for 3 days with MS1 mouse endothelial cell line. (B) RT-PCR analysis of smooth muscle markers' mRNA in human mesenchymal stem cells, human aortic vascular smooth muscle cells (CRL-1999), and human dermal fibroblasts (CRL-2575). Water was used as negative control. (C-F) Protein expression of smooth muscle markers in hMSCs was confirmed by immunohistochemistry (α-smooth muscle actin [C], SM22α [D], desmin [E], and NG2 [F], yellow; DAPI, blue). Scale bar represents 50 μm.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/111/9/10.1182_blood-2007-10-118273/4/zh80080818120001.jpeg?Expires=1723328731&Signature=ExjoyUA5Umv7dWw48Wcnzfof4Uy6PG6Z5CxdFtlShPSdNOatlcbL08GE4hrJl-vM3S3x01JP6QcgEqupkGLVv7yNQwC0PTjPGrqdVUkqmEyYOic85S3KyNVseuq0EXfuSACr79HVz-fUGgUp91gWY9pV1kmi7o9hO7GsXY5UL1jZzqgfMnfLhwrMcSjLEehjZRdG9JX3oMGo1RF-n5ihUiGyBb8L5OBgsaBFPObgE5~CC-mNY-eTVEOqkLx6ohiKS2Hi2lr3iLxruR3jjXK~dkH1YSDB-mz~wV-rT3lEv1-ZzUm9MjbTotIcfDzrOL-8aVfx4Laa4MnHM9sTeqiLIw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transcriptional profile of endothelial and smooth muscle markers in hMSCs. (A) RT-PCR analysis of endothelial markers (VE-cadherin and PECAM1) in hMSCs at baseline and in hMSCs cocultured for 3 days with MS1 mouse endothelial cell line. (B) RT-PCR analysis of smooth muscle markers' mRNA in human mesenchymal stem cells, human aortic vascular smooth muscle cells (CRL-1999), and human dermal fibroblasts (CRL-2575). Water was used as negative control. (C-F) Protein expression of smooth muscle markers in hMSCs was confirmed by immunohistochemistry (α-smooth muscle actin [C], SM22α [D], desmin [E], and NG2 [F], yellow; DAPI, blue). Scale bar represents 50 μm.
