Enrichment profiles of histone modifications in the βh1 and β^major/β^S globin gene. Allele-specific analyses were conducted in ΔLCR (Hbb^D)/WT (Hbb^S) heterozygous mouse spleen cells. (A) The βh1 and the β^major/β^S globin genes are shown. Positions of PCR amplicons (a-g) are shown as bars. Primers and restriction enzymes used in this analysis are described in Document S1. Enrichment of modifications relative to myoD1 in the WT (▩) and ΔLCR (□) alleles is shown. (B) H3 K79 dimethylation. (C) H3 K4 dimethylation. (D) H3 K36 trimethylation. Each bar represents the average of multiple experiments. Error bars represent SD.