Neutralizing anti-VEGF antibodies suppress 14,15-EET–induced HDMVEC migration and tube formation in vitro and Matrigel plug angiogenesis in vivo. Quiescent HDMVECs were treated with neutralizing anti-VEGF antibodies (3 μg/mL) for 30 minutes at 37°C followed by washing with medium 131. The cells were then subjected to 14,15-EET (0.1 μM)–induced migration (A,B) or tube formation (C,D) in the presence and absence of 3 μg/mL neutralizing anti-VEGF antibodies. (E,F) C57BL/6 mice were injected subcutaneously with 0.5 mL Matrigel premixed with vehicle or 50 μM 14,15-EET with and without 3 μg/mL neutralizing anti-VEGF antibodies. One week later, the animals were killed and the Matrigel plugs were harvested from underneath the skin and analyzed for either hemoglobin content with Drabkin reagent or immunostained for CD31 expression using anti-CD31 antibodies. Preimmune serum was added to Ad-GFP– and Ad-GFP + 14,15-EET–treated cells or mice. The values in the bar graphs in panels B, D, and E are the means plus or minus SD of 3 independent experiments or 4 animals. *P < .01 vs control; **P < .01 vs 14,15-EET.