Adenovirus-mediated expression of dnSrc suppresses 14,15-EET–induced HDMVEC migration and tube formation in vitro and Matrigel plug angiogenesis in vivo. (A,B) HDMVECs were transduced with Ad-GFP or Ad-dnSrc at an MOI of 80, quiesced, and subjected to 15(S)-HETE–induced migration (A,B) or tube formation (C,D). (E,F) C57BL/6 mice were injected subcutaneously with 0.5 mL Matrigel premixed with vehicle or 50 μM 14,15-EET with and without Ad-GFP or Ad-dnSrc (5 × 109 pfu/mL). One week later, the animals were killed and the Matrigel plugs were harvested from underneath the skin and analyzed for either hemoglobin content with Drabkin reagent or immunostained for CD31 expression using anti-CD31 antibodies. The values in the bar graphs in panels B, D, and E are the means plus or minus SD of 3 independent experiments or 4 animals. *P < .01 vs Ad-GFP; **P < .01 vs AD-GFP + 14,15-EET.
