Adenovirus-mediated expression of dnSrc inhibits 14,15-EET–induced STAT-3 phosphorylation in HDMVECs. HDMVECs that were transduced with Ad-GFP or Ad-dnSrc at an MOI of 80 were treated with and without 14,15-EET (0.1 μM) for the indicated times, and cell extracts were prepared and analyzed by Western blotting for pSTAT-3 using its phosphospecific antibodies. The blot was reprobed sequentially with anti–STAT-3 antibodies for normalization and anti-Src antibodies for overexpression of Src.