Figure 2
Validation of expression levels of selected FLT3-ITD classifier genes by Q-RT-PCR. Heatmap representation of gene-expression levels of CFH, SOCS2, STON2, and MYO1B, quantified by microarray or Q-RT-PCR, measured for 20 NK-AML samples (10 each from training and test sets). Samples are ordered by PAM probabilities of FLT3-ITD classification. Q-RT-PCR expression levels shown are normalized to GAPDH levels, and both microarray and Q-RT-PCR expression ratios are log2-transformed and mean centered (ratio color scale indicated). Correlations (r) between microarray and Q-RT-PCR measured expression levels are indicated.
