GSD-Ib neutrophils display increased rate of apoptosis. Peritoneal neutrophils were isolated from 6- to 7-week-old unaffected (+/+) and GSD-Ib (−/−) mice during thioglycollate-elicited peritonitis. (A) Representative flow cytometric analysis of neutrophil annexin V (x-axis) binding and PI (y-axis) uptake. Numbers on the plots are percentages of total cells. (B) Quantification of annexin V–positive neutrophils determined by immunofluorescence staining. (C) Quantification of caspase-3–positive neutrophils determined by immunofluorescence staining. (D) The DEVD-cleaving activity of active caspase-3 in protein extracts of peritoneal neutrophils. Data represent the means (± SEM) of 3 independent experiments. **P < .001.