Increase in the expression of ER chaperones in neutrophils of GSD-Ib mice. Peritoneal neutrophils were isolated from 6- to 7-week-old unaffected (+/+) and GSD-Ib (−/−) mice during thioglycollate-elicited peritonitis. (A) Hema 3–stained cytospins of peritoneal neutrophils. (B) Western-blot analysis of protein extracts of peritoneal neutrophils using antibodies against gelatinase, Gr-1, β-actin, or GAPDH. Each lane contains 50 μg protein. (C) Western-blot analysis of protein extracts of neutrophils using antibodies against GRP78, GRP170, PDI, or β-actin. Each lane contains 50 μg protein.