Sorafenib lowers expression of cell surface molecules on DCs stimulated with LPS. DCs were generated by incubating adherent monocytes with GM-CSF and IL-4. On day 5 of culture, cells were exposed for 24 hours to sunitinib (A,B) or sorafenib at the indicated concentrations (C,D). LPS (100 ng/mL) was added as a stimulus to the culture media for the last 24 hours (B,D). In addition, to analyze the effects of sorafenib on the phenotype of mature DCs sorafenib was added to the culture medium after the activation of cells with LPS for 24 hours (E). The changes in phenotype of DCs by sorafenib treatment were analyzed by flow cytometry after staining with Abs against CD1a, CD80, CD86, CD83, DC-SIGN, or CCR7. Shaded histograms represent isotype control and open diagrams staining with the specific antibody. The numbers represent mean fluorescence intensity. One representative experiment of at least 3 is shown.