Soluble biomarkers in cGVHD. Soluble factors were tested for (A) sBAFF, (B) sIL-2Rα, (C) IL-6, (D) TGF-β, (E) IFN-α, (F) MCP-1, (G) PDGF-AA, and (H) PDGF-BB by ELISA. Patients with early onset (3-8 months) cGVHD (▴) were compared with 6-month controls (■), patients with late onset (≥ 9 months) cGVHD (♦) were compared with 12-month controls (▾). (I) sCD13 activities were analyzed by the protocol described under “Methods.” (J) Absolute eosinophil counts were from center-derived complete peripheral blood counts. Bars indicate mean values for each group. ***P < .001; **P < .01; *P < .05. NS indicates not significant.