Figure 7
![Figure 7. Molecular analysis for Phd2 CKO and Phd1/3 DKO mice. (A) Autoradiography of [35S]methionine-labeled PHD1, 2 and 3 produced by IVTT. Free, unincorporated [35S]methionine. (B) Western blotting analysis for PHD1, -2, and -3 in various tissues from wild-type adult mice. Equal amounts of IVTT-produced PHD1, -2, and -3 proteins were loaded as controls. Note PHD1 had 2 bands because of alternative translational initiation. (C) Anti-PHD2 Western blots for Phd1/3 DKO tissues. (D) Anti-HIF-1α and HIF-2α Western blots for Phd2 CKO and Phd1/3 DKO mice. (E,F) Q-PCR analysis for Epo mRNA. f/f, Phd2f/f; CKO, Phd2 CKO; WT, wild-type; DKO, Phd1/3 DKO. n = 6. (F) The scale in panel E was reduced. **P < .01; *P < .05. Error bars represent SEM.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/111/6/10.1182_blood-2007-09-114561/6/zh80040815160007.jpeg?Expires=1724232589&Signature=pYz0pV66mmQ~QGnQ8YyrPeilzAyvS~D1IAA~ssxkhl4TZEeu7OaI1ohKe0EIaxB1mmgQ7gHU-P33t--g0Navdr41NGSKkV4n816qBQkXZUtWHzE6b5GoO60alCNlZTeDz~HwbYARo6eH7SxJUUtF4ZCNz9ewNTNdmcJM23IfgBzddAU~v2XbMMApO4XeXZN8LF7F1ISaGrHegT04PQC-Y6H2gQk6od40HTXTxDVo0-tmW5ysurlG4EJZuE42B9QSDtIiCwSCU3HmaBLFn1eAUNWW3hw4Aca8MA-cQhHuB-h7YWl9eczGISvm-Q4QRz-ucD9oieJha4EWi2i7naypfw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Molecular analysis for Phd2 CKO and Phd1/3 DKO mice. (A) Autoradiography of [35S]methionine-labeled PHD1, 2 and 3 produced by IVTT. Free, unincorporated [35S]methionine. (B) Western blotting analysis for PHD1, -2, and -3 in various tissues from wild-type adult mice. Equal amounts of IVTT-produced PHD1, -2, and -3 proteins were loaded as controls. Note PHD1 had 2 bands because of alternative translational initiation. (C) Anti-PHD2 Western blots for Phd1/3 DKO tissues. (D) Anti-HIF-1α and HIF-2α Western blots for Phd2 CKO and Phd1/3 DKO mice. (E,F) Q-PCR analysis for Epo mRNA. f/f, Phd2f/f; CKO, Phd2 CKO; WT, wild-type; DKO, Phd1/3 DKO. n = 6. (F) The scale in panel E was reduced. **P < .01; *P < .05. Error bars represent SEM.
