Figure 2
Assessment of eosinophil distribution, eotaxin, and CCR3 expression in Pirb−/− mice. (A) Quantification of eosinophil distribution in the gastrointestinal tract of naive wild-type (WT) and Pirb−/− mice. Eosinophils were identified by MBP-stained sections and quantified using digital morphometric analysis. Data represent means plus or minus SD of 4 or 5 random sections per mouse for 6 or 8 animals per group; *P < .05; **P < .01 when comparing Pirb−/− and wild-type mice. Eso indicates esophagus. (B) The levels of eotaxin-1 and eotaxin-2 were assessed in small intestinal homogenates of WT and Pirb−/− mice using a commercial ELISA. Data represent mean plus or minus SD of homogenates from 8 mice. ns indicates nonsignificant. (C) The expression of CCR3 as assessed by FACS on intestinal eosinophils from WT and Pirb−/− mice. MFI indicates mean fluorescent intensity. Data represent n = 4 experiments. Error bars represent SD.

Assessment of eosinophil distribution, eotaxin, and CCR3 expression in Pirb−/− mice. (A) Quantification of eosinophil distribution in the gastrointestinal tract of naive wild-type (WT) and Pirb−/− mice. Eosinophils were identified by MBP-stained sections and quantified using digital morphometric analysis. Data represent means plus or minus SD of 4 or 5 random sections per mouse for 6 or 8 animals per group; *P < .05; **P < .01 when comparing Pirb−/− and wild-type mice. Eso indicates esophagus. (B) The levels of eotaxin-1 and eotaxin-2 were assessed in small intestinal homogenates of WT and Pirb−/− mice using a commercial ELISA. Data represent mean plus or minus SD of homogenates from 8 mice. ns indicates nonsignificant. (C) The expression of CCR3 as assessed by FACS on intestinal eosinophils from WT and Pirb−/− mice. MFI indicates mean fluorescent intensity. Data represent n = 4 experiments. Error bars represent SD.

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