Scl occupies the Runx3 promoter and a Runx1 candidate enhancer in vivo. Real-time PCR analysis of ChIP performed with anti Scl (A,B) and antiacetylated histone 3 (C,D) antibodies. The y-axis represents enrichment over that obtained with a rabbit IgG antibody. The x-axis represents the coordinates at the mouse Runx1 (A,C) and Runx3 (B,D) gene loci where the conserved E-boxes are depicted by numbered arrows. NT indicates not tested. Error bars represent SD.