c-Abl is required for WAVE2 localization independent of Rac1/Cdc42 activation. (A) Jurkat T cells were treated with or without 10 μM of imatinib mesylate for 1 hour, and cells were stimulated with C305 for indicated times, lysed, and affinity precipitated with GST-Pak-CRIB, followed by immunoblotting with antibodies to Rac1 and Cdc42. (B) Jurkat T cells transfected with control vector or shAbl-3 (green) were conjugated with SEE pulsed Raji B cells (blue). Conjugates were fixed and labeled for WAVE2 (red).