Cytokine profiles from transduced DC cultures. (A) DCs were transduced with lentivectors encoding activators and GFP. Culture supernatants were collected 6 days after transduction and enzyme-linked immunoassays (ELISAs) were performed to quantify the indicated cytokine levels. Results from ELISAs are shown as column graphs representing the means from 6 independent experiments, together with error bars (standard deviations [SD]). Relevant statistical comparisons between indicated samples and GFP-expressing DCs are shown within each graph. IFN-β level in LPS-treated DCs is shown on top of the column; B indicates means below detection limit; *, significant differences (P < .05); **, very significant differences (P < .01). (B) IL-10 levels were quantified by ELISA from supernatants of DC cultures from the indicated mouse strains (TRIF-, MYD88-, IFNAR-, WT) transduced with lentivectors encoding only GFP, IRF3 2D, or IRF3ΔC, as indicated at the bottom of the graph. Mean IL-10 levels from 3 experiments are represented as columns, together with error bars (SD). Dotted line indicates the ELISA sensitivity level. IRF and ΔC represent IRF3 2D and IRF3ΔC mutants. LPS represent LPS-treated DCs. (C) Flow cytometric histograms are shown from intracellular TGF-β staining in DCs transduced with the indicated lentivectors, or treated with LPS.