American Society of Hematology

Figure 1

$Figure 1. Delayed mobilization of CD34+ myeloid progenitors in EAE mice. (A) The percentage of CD34+ cells was assessed by flow cytometry on peripheral blood mononuclear cells (PBMCs) obtained from control mice or from EAE mice killed on day (d) 21 (first relapse), d28 (chronic phase), or d78 (chronic phase) after immunization (p.i.). The percentage of blood CD34+ cells was not significantly changed in EAE d21 mice versus controls (0.97% vs 0.81%; left panel). However, it significantly increased in EAE d28 mice (2.05%) or EAE d78 mice (2.2%) compared with controls (left panel). Such an increase could be similarly evidenced when considering the percentage of CD34+ cells in the CD11b+ fraction of PBMC (right panel). **P < .01, ***P < .001, Student t test. (B) Data show representative dot plots obtained by FACS analysis of PBMCs obtained from EAE or control mice.$

Delayed mobilization of CD34⁺ myeloid progenitors in EAE mice. (A) The percentage of CD34⁺ cells was assessed by flow cytometry on peripheral blood mononuclear cells (PBMCs) obtained from control mice or from EAE mice killed on day (d) 21 (first relapse), d28 (chronic phase), or d78 (chronic phase) after immunization (p.i.). The percentage of blood CD34⁺ cells was not significantly changed in EAE d21 mice versus controls (0.97% vs 0.81%; left panel). However, it significantly increased in EAE d28 mice (2.05%) or EAE d78 mice (2.2%) compared with controls (left panel). Such an increase could be similarly evidenced when considering the percentage of CD34⁺ cells in the CD11b⁺ fraction of PBMC (right panel). **P < .01, ***P < .001, Student t test. (B) Data show representative dot plots obtained by FACS analysis of PBMCs obtained from EAE or control mice.

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