ChIP and coIP analysis of T-ALL cell lines LOUCY and SKW3. (A) ChIP analysis of SKW3, LOUCY, and LOUCY 4 days after electroporation with siRNA SET exon 5, for promoter sequences of HOXA1, HOXA3, HOXA9, HOXA10, and HOXA11. The amount of bound DNA was calculated relative to the 5% input DNA in anti-NUP214 and anti-acetyl histone H3 immunoprecipitates, whereas no antibody and anti-FLAG immunoprecipitates were used as negative control. Error bars represent SEM. (B) Western blot analysis of NUP214 and PP32 immunoprecipitates of the cell line LOUCY using anti-SET. (C) Similar Western blot analysis as in panel B for CRM1 and DOT1L immunoprecipitates in LOUCY. (D) Western blot analysis of NUP214 immunoprecipitates of the cell line LOUCY using anti-AF10.