siRNA knockdown of SET and SET-NUP214 in the LOUCY T-ALL cell line. (A) SET expression using Western blot analysis 4 days after electroporation with the following conditions: no siRNA, control siRNA, siRNA SET exon 8, or siRNA SET exon 5. (B) Relative SET and SET-NUP214 expression by RQ-PCR after 2, 4, and 7 days for conditions as mentioned in panel A. (C) Relative expression of all members of the HOXA clusters by RQ-PCR (except for HOXA11 and HOXA13) after 4 days for conditions as mentioned in panel A. (D) Optical density (OD) values relative to control cells without pulse after 7 days for conditions as mentioned in panel A. (E) Total cell numbers after 7 days for conditions as mentioned in panel A. (F) Percentage of cell death relative to control cells without pulse after 7 days for conditions as mentioned in panel A. Error bars visualize the standard error of the mean (SEM). (G) FACS analysis 6 days after electroporation with either no siRNA, siRNA SET exon 8, or siRNA SET exon 5 for TCRγδ and membrane CD3. The percentage of cells positive for TCRγδ or mCD3 expression are visualized in quadrant 4.