SET-NUP214 fusion transcript in T-ALL. (A) RT-PCR analysis using SET- and NUP214-specific primers and GAPDH primers as internal control, reveals a specific SET-NUP214 fusion gene in T-ALL patient nos. 125, 126, and 120; the patient with AUL; and the T-ALL cell line LOUCY. NUP214-ABL1 fusion was detected in patient nos. 120 and no.88 and in the T-ALL cell line PEER (B) Sequence analysis confirmed an identical fusion between exon 7 of SET and exon 18 of NUP214 in all SET-NUP214⁺ patients with T-ALL, the patient with AUL, and the LOUCY cell line. (C) Western blot analysis of T-ALL cell lines revealed a SET-NUP214 fusion in the cell line LOUCY. (D) At the protein level, the breakpoints are situated in the acidic tail of SET and the coiled-coil domain of NUP214.