Ascites from tumor-bearing Type I NKT cell–deficient mice have elevated levels of myeloid suppressor (CD11b⁺Gr1⁺) cells. (A) NS0-V or NS0-CD1 tumor-bearing BALB/c wildtype, CD1KO, and Jα18KO mice were euthanized, and ascites tumor cells were harvested and stained for surface expression of CD44, B220, DX5, TCRβ and CD4, TCRβ and CD8, or CD11b and Gr1 and analyzed by flow cytometry. Each bar corresponds to the mean percentage of positive cells for a specific antigen obtained from 3 individual mice in each group (+ SD). (B) Splenocytes harvested from the above groups of mice were stained for Gr1 and CD11b. A representative FACS analysis of 3 individual mice is shown. Numbers on the plots indicate the percentage of total cells in that quadrant.