Levels of pro- and anti-inflammatory cytokines are indicators of murine B cell lymphoma growth in vivo. Splenocytes from BALB/c wildtype, CD1KO, and Jα18KO mice inoculated with NS0-V or NS0-CD1 cells were collected 25 (A) or 50 (B) days later and cocultured with irradiated tumor cells. The culture supernatants were tested for the indicated cytokines by ELISA. Each bar represents the cytokine production from individual mice (n = 6 from 2 independent experiments). No bar in a group indicates that the cytokine was below the level of detection. In the day 50 NS0-CD1 tumor-bearing Jα18KO mouse group; 3 mice were dead by day 50 (#) and hence, cytokine production could not be measured. (C) BALB/c mice were inoculated with the tumors as above, and on days 0, 5, 10, 20, 30, 40, and 50, mice were bled and the serum assayed for TGFβ by ELISA. Error bars represent SD. In panels A and B, the asterisk indicates a significant P value (< .025) as determined by the Kruskal-Wallis test for the comparisons made between the indicated groups.