Interaction of ADAMTS13 with VWF glycan mutants. (A) ADAMTS13 cleavage of recombinant VWF was repeated in the absence of 1.5 M urea and the extent of cleavage analyzed by CBA. Shown for comparison is the cleavage of recombinant VWF in the presence of 1.5 M urea. Cleavage of wtVWF and the N1515Q mutant in the absence of urea is represented by a single line. (B) Recombinant VWF was coated on to MaxiSorp plates at a final concentration of 7.5 μg/mL and incubated with decreasing concentration of ADAMTS13 and bound protein detected with pAb Tsp2-4. Error bars represent mean and SD.