Supernatants from reovirus-infected CBMCs induce CXCL8-dependent NK cell migration. CBMCs were infected with reovirus or UV-inactivated reovirus, or with medium (med) as a negative control for 24 hours. The resultant supernatants were left untreated, or treated with an anti-CXCL8 (4 μg/mL) or isotype control mAb before being placed in the bottom chamber of 96-well chemotaxis assays. FACS-isolated CD56⁺ CD3⁻ NK cells were placed in the top chamber, and chemotaxis was assessed after 2 hours. Mean migration to supernatants: reovirus, 17.2%; UV-reovirus, 12.3%. Graph shows the mean (± SEM) of the fold increase in chemotaxis over medium of 4 experiments using different CBMC cultures. *P < .05 compared with CBMC medium; †P < .05, ††P < .01, compared with no treatment.