NK cells but not CD56⁺ T cells migrate in a CXCL8-dependent manner. Supernatants from CBMCs stimulated with poly(I:C) [p(I:C)] or medium control (med) were treated with an anti-CXCL8 or isotype control mAb previous to placement in the bottom chamber of 96-well chemotaxis assays. Poly(I:C) was added to medium alone to control for potential chemotactic/chemokinetic effects of the poly(I:C). FACS-isolated CD56⁺ CD3⁻ NK cells (top) or CD56⁺ CD3⁺ T cells (bottom) were placed in the top chamber. CXCL8 (30 ng/mL) was used as a positive control for anti-CXCL8 mAb inhibition of chemotaxis during the 2-hour migration. Mean migration to poly(I:C)-CBMC supernatants: NK cells, 14.8%; CD56⁺ T cells, 16%. Graphs represent the mean (± SEM) of the fold increase in chemotaxis over medium of 2 to 6 separate experiments, except for CD56⁺ CD3⁺ T-cell migration to CXCL8 (n = 1). ***P < .001 compared with CBMC medium; †††P < .001 compared with no treatment.