Supernatants from poly(I:C)-stimulated CBMCs induce CD56⁺ cell and T-cell migration. Supernatants from CBMCs incubated with poly(I:C) (p(I:C)) or medium (med) for 24 hours were placed in the bottom chambers of 24-well chemotaxis assays. CXCL10 alone (50 ng/mL) was used as a positive control, and medium containing poly(I:C) was used as a control for potential chemotactic/chemokinetic effects of the poly(I:C). Human nylon wool–purified peripheral blood lymphocytes were placed in the top chamber. After 2 hours, flow cytometry was performed to analyze the cell subsets migrating into the bottom chamber. Graphs show the mean percentage chemotaxis of CD56⁺ cells and T cells from each of 3 separate blood donors performed in duplicate, where each blood donor population migrated toward supernatants from different CBMC cultures (n = 3). Mean migration to poly(I:C)-CBMC supernatants: CD56⁺ cells, 25.4%; CD8⁺ T cells, 3.3%; and CD8⁻ T cells, 4.3%.