Inhibition of RAC1 activity decreases ROS production in 32D/ITD cells. (A) Inhibition of ROS production by a RAC1 inhibitor (NSC23766). RAC1 inhibitor was used at the concentrations of 400 μM and 600 μM overnight. The inhibition was represented by the percentage fluorescence unit, compared with the untreated cells. (B) 32D/ITD cells transfected with dominant negative RAC1 (N-17) resulted in significant decrease in ROS production. Transfection with vector controls show no significant changes in ROS. The error bars represent plus or minus SEM.