Examples of IHC and FISH analysis on t(11;14)-negative MCL. Upper panels show IHC findings in cases 2 (A), 3 (B), 6 (C), and 8 (D) using antibodies against cyclin D1, D2, D3, and cyclin E, respectively. Note a t(11;14)-independent expression of cyclin D1 in case 2 (A), expression of cyclin D2 in case 3 with a t(2;12)(p11;p13)IGK/CCND2 rearrangement (B), expression of cyclin D3 in case 6 with a t(6;14)(p21;q32)IGH/CCND3 aberration (C), and expression of cyclin E in case 8 negative for D-type cyclins. Lower panels are examples of FISH performed in cases 3 (E), 6 (F), 7 (H, inset) and 8 (G,H). Applied probes were CCND2 break-apart (E), CCND3 break-apart (F), LSI IGH (G), and 3′IGH (SpectrumGreen)/LSI N-MYC (SpectrumOrange) (H). Note a split of signals from probes flanking CCND2 in case 3 (E), translocation of CCND3 on a der(14q) in case 6 (F), split of LSI IGH signals heralding a cryptic t(2;14)(p24;q32) in case 8 (G), a fusion (red LSI N-MYC/2p24 and green 3′IGH/14q32) signal on a der(14) in case 8 with t(2;14) (H), and the same FISH pattern detected in interphase cells from case 7 (H, inset).