Gossypol is more hepatotoxic than apogossypol. Balb/c mice were orally administered apogossypol, gossypol, or vehicle control at a daily dose of 120 μmol/kg 5 times weekly for 3 weeks (6 mice per group). (A) Serum levels of ALT and AST were measured at conclusion of treatment (mean ± SD). (B) H&E histology of liver sections is presented. Note area of necrosis in gossypol-treated animal shown. Representative of 3 of 6 mice evaluated for each treatment group. (C) TUNEL-stained sections of liver are shown (representative of 3 of 6 mice for each group). Size bars represent 100 μM.