Inhibition of PI3-K/Akt but not mTOR pathway decreases telomerase activity in MCL. Granta 519 and SP-53 cells were treated with the PI3-K/Akt inhibitors, 50 μM LY294002 (Ly), 20 μM SH-5, and 0.1 μM rapamycin (Rapa), for 7 and/or 24 hours and then analyzed for telomerase activity by TRAP assay. TL indicates telomerase ladder; ITAS, internal telomerase assay standard.