CCL5 induces formation of the eIF4F initiation complex. (A) Activated PB T cells were either left untreated or treated with 10 nM CCL5 for 30 minutes, lysed, and immunoprecipitated with either anti-eIF4E or anti-eIF4G antibodies. Samples were resolved by SDS-PAGE and immunoblotted with anti-eIF4E and anti-eIF4G antibody. Whole molecule mouse or rabbit IgG was used as control. (B) Cells were either left untreated or treated with 10 nM CCL5 for the indicated times; then lysates were resolved by SDS-PAGE and immunoblotted with anti–phospho-eIF4E (Ser 209) antibody. Membranes were stripped and reprobed for eIF4E as a loading control. Data are representative of 2 independent experiments plus or minus SD. Values denoting the extent of phosphorylation are shown in the right-hand panel.