Purity of human and murine neutrophils. (A) Analysis by FACS of BMN (left panel) and PEN (right panel) purity using PE-labeled anti–Ly-6G mAb and FITC-labeled antineutrophil mAb (7/4). (B) Analysis of BMN purity using FITC–anti-CD11c and PE–anti-F4/80 mAbs (white histograms; gray histograms correspond to isotype control mAbs); similar data were obtained for PENs. (C,D) Analysis by FACS of human neutrophil purity using FITC–anti-CD13, anti-CD11b, and anti-CD14 mAbs (white histograms; gray histograms correspond to isotype control mAbs (C) and using the FSC/SSC parameters (D).