Murine crlr overexpression rescues the vascular phenotype in zebrafish crlr morphants. Murine crlr mRNA was injected in VEGFR2:G-RCFP crlr morphants, and axial vasculature of the trunk was examined under an epifluorescence microscope at 26 hpf. The reduction in size of DA (red bar) and the delay in ISV development observed in crlr morphants (B) are rescued by murine crlr overexpression (C). (A) control VEGFR2:G-RCFP embryo. PCV is indicated by the white bar. The percentage of embryos showing normal ISV development in the different experimental groups is shown in panel D. Data are the mean plus or minus SD of 2 independent experiments with 15 embryos per group. *Statistically different from the “morphant” group; P < .02, Student t test.