Gene expression of TNF family BAFF receptors, BCMA, TACI, and BAFF-R, is decreased in preterm neonatal cord blood–derived B cells compared with adult B cells. Real-time quantitative RT-PCR was performed on RNA obtained from freshly isolated naive B cells. The amount of mRNA for each gene of interest was normalized relative to β-actin mRNA, and the graphs were generated with the relative values obtained after normalization. Data are shown as mean (± SEM) of 10 independent experiments using 10 independent adult (AB), cord (CB), or preterm cord (PreCB) blood samples with each assay performed in triplicate. Statistically significant differences are shown. *P < .001; **P ≤ .005.