Effects of TGF-β1 on phenotypic markers of M-DCs. CB monocytes were cultured with M-CSF and IL-4 ± TGF-β1 for 5 to 6 days. LPS was added on the last day of cell culture. Cells were stained with CD1a, CD80, CD86, CD207 (langerin), HLA-DR, and CD83. Gray line designates isotype control. The black area represents indicated molecules. The mean fluorescence intensities of indicated molecules are shown on the upper right corner of the histograms. Results are representative of 3 to 5 independent experiments.