Figure 2
FcαRI-induced apoptosis is mediated by FcRγ ITAM. Induction of apoptosis in RBL-2H3 cells transfected with transmembrane mutated FcαIRR209L (A), chimeric FcαRIR209L/γ (B), or chimeric ITAM-mutated FcαRIR209L/γY268/278F (C). Cells were treated with 10 μg/mL A77 or irrelevant control (320) Fab for 24 hours before annexin V/PI staining (left panel) or analysis of hypoploid nuclei (middle panel) as described in “Materials and methods.” The percentage of apoptotic (annexin V/PI double-positive) cells is shown (right panel). Values are the percentages of double-positive cells and are means ± SD of at least 3 separate experiments performed in duplicate. *P < .01.

FcαRI-induced apoptosis is mediated by FcRγ ITAM. Induction of apoptosis in RBL-2H3 cells transfected with transmembrane mutated FcαIRR209L (A), chimeric FcαRIR209L/γ (B), or chimeric ITAM-mutated FcαRIR209L/γY268/278F (C). Cells were treated with 10 μg/mL A77 or irrelevant control (320) Fab for 24 hours before annexin V/PI staining (left panel) or analysis of hypoploid nuclei (middle panel) as described in “Materials and methods.” The percentage of apoptotic (annexin V/PI double-positive) cells is shown (right panel). Values are the percentages of double-positive cells and are means ± SD of at least 3 separate experiments performed in duplicate. *P < .01.

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