Expression of the IgH^P5ki allele in lymphoid and dendritic cells. (A) GFP expression in different hematopoietic cell types. Bone marrow (BM) cells and splenocytes (SPL) of 6-week-old IgH^P5ki/+ (ki/+) and IgH^P5ki/P5ki (ki/ki) mice were stained with the indicated antibodies, and GFP expression of the IgH^P5ki knock-in alleles (gray area) was displayed for c-Kit⁺B220⁺ pro-B cells (BM), B220⁺CD19⁺ B lymphocytes (BM), CD4⁺ and CD8⁺ T cells (SPL), NK1.1⁺TCRβ⁻ NK cells (SPL), CD11c⁺CD8α⁺ and CD11c⁺Mac1⁺ DCs (SPL), Gr1⁺Mac1⁺ granulocytes (BM), M-CSFR⁺Mac1⁺monocyte/macrophages (BM), and Ter119⁺ erythroblasts (BM). Black lines indicate the absence of GFP expression in the corresponding wild-type cells. (B) Expression of the IgH^P5ki allele in uncommitted progenitors. GFP expression is displayed for the Lin⁻IL7Rα⁻Sca1^hic-Kit^hi MPPs and Lin⁻IL7Rα⁺Sca1^loc-Kit^lo CLPs in IgH^P5ki/+ (gray area) and wild-type (black line) BM. The percentage of GFP⁻ and GFP⁺ cells is shown for MPPs and CLPs of the IgH^P5ki/+ mouse.