Expression level on a per-gene basis in ENH-right and ENH-left constructs is integration site dependent and higher than that of the EPS-ISC construct. The data represent a summary of different real-time RT-PCR experiments, analyzed on a per-gene basis. The RNA expression in nonstimulated B cells from the EPS21 transgenic line was considered to be 1, and all samples from different transgenic lines and stimulation conditions were compared to it. The average values from 6 independent experiments (EPS21 line) or 2 independent experiments (all other transgenic lines) are shown and SDs are indicated. When compared with EPS-ISC, the stimulated responses of R7, R22, R27, and L12, but not L26, were significantly different (P < .05 or lower). The LPS plus IL-4 or anti-CD40 plus IL-4 responses of R7 and R27 were not significantly different from that of L12, but responses in R22 were (P < .01 and P < .001, respectively).