Figure 3
Figure 3. The ENH-right transgene expression is higher than that of the enhancer-less construct and is induced by cytokines. RT-PCR was performed with RNA from 3 different transgenic ENH-right lines (R7, lanes 1-4; R22, lanes 5-8; and R27, lanes 9-12) and the EPS21 transgenic line (lanes 13-16). The experiment is performed in the same manner as in Figure 2. (A) Results from RT-PCR. (B) The same samples are quantified by real-time RT-PCR. All samples are compared with nonstimulated cells of EPS21.

The ENH-right transgene expression is higher than that of the enhancer-less construct and is induced by cytokines. RT-PCR was performed with RNA from 3 different transgenic ENH-right lines (R7, lanes 1-4; R22, lanes 5-8; and R27, lanes 9-12) and the EPS21 transgenic line (lanes 13-16). The experiment is performed in the same manner as in Figure 2. (A) Results from RT-PCR. (B) The same samples are quantified by real-time RT-PCR. All samples are compared with nonstimulated cells of EPS21.

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