Genotyping analysis of B/NK/myeloid individual clones derived from PV and IMF purified CD34⁺CD38^minus]. (A) Immunophenotypic analysis of B/NK/myeloid clones from a healthy bone marrow, an IMF patient, and a PV patient. The isotype control scattergrams are shown in the top panel. The B, NK, and myeloid potentials were assessed by the presence of CD19⁺, CD56⁺, and CD15⁺ cells, respectively, after 5 weeks of B, NK, and myeloid differentiation condition culture. The JAK2 G1849T homozygous sequence traces of the IMF and the PV clones are shown. Red arrows indicate the mutant T peak. (B) Genotype analysis of single CD34⁺CD38⁻ cell culture-derived clones from 3 IMF (i-iii) and 5 PV patients (iv-viii) with respect to their monopotent B, NK, myeloid (M), bipotent B/M, B/NK, M/NK, or tripotent B/NK/M immunophenotypic characterization (clone type).