Diminished nuclear RelB during d.a.MKK6-induced maturation leads to enhanced DC activation. TA-transduced CD34⁺ cells were cotransduced with control vectors (ctrl), d.a.MKK6-NGFR + ctrl (MKK6), d.a.MKK6-NGFR + p100ΔN-GFP, ctrl + p100ΔN-GFP (p100ΔN), or d.a.MKK6-NGFR + IκB-SR-GFP. Genes were coinduced in day 8 LCs by DOX (2 μg/mL) for 48 hours. (A) GFP⁺NGFR⁺ cells were gated for CD1a expression and analyzed by FACS for the expression of CD86, CD80, CD83, and CD40. Bar diagrams depict mean percentages ± SD of 4 independent experiments (paired Student t test was performed; horizontal bars cluster the statistical comparisons, *P < .05, **P < .01). (B) CD1a⁺GFP⁺NGFR⁺ cells were sorted and subjected to an allo-MLR. Diagrams represent 1 representative experiment. (C) GFP⁺NGFR⁺ cells were gated for CD1a expression and analyzed by FACS for MHC II expression. Bar diagram depicts mean percentages ± SD of 4 independent experiments.