Conditional expression of RelB in LCs does not induce maturation. (A) Representative Western blot analysis for RelB. CD1a⁺NGFR⁺ LCs induced to express d.a.MKK6-NGFR or control vector in the presence of DOX for 48 hours were sorted, and total-cell lysates were prepared. As control, lysates of CD1a⁺ LCs grown in the absence of DOX were used. Actin detection was the loading control. (B) (Left) CD1a⁺NGFR⁺ LCs induced to express RelB-NGFR were sorted, and RelB expression was analyzed by Western blot. As control, lysates of CD1a⁺ RelB-transduced LCs grown in the absence of DOX or of CD1a⁺ mock-infected LCs were used. Actin detection was the loading control. (Right) Control vector (ctrl), RelB-NGFR, or d.a.MKK6-NGFR was induced in immature LCs by DOX (2 μg/mL), and gated NGFR⁺ cells were analyzed for CD86, CD83, and CD40 by FACS after 48 hours. Bars represent the mean percentages ± SD of 3 independent experiments. P values (**P < .01, ***P < .001) were determined for control versus MKK6 or control versus RelB.