d.a.MKK6 selectively induces IL-1β and IL-6 production of CD34⁺-cell–derived LCs. CD34⁺ cells transduced with TA and d.a.MKK6-NGFR (or control) were differentiated to LCs. On day 8, gene expression was induced by DOX addition (2 μg/mL), and sorted CD1a⁺NGFR⁺ cells were replated for cytokine production in GM-CSF (100 ng/mL), FL (50 ng/mL), SCF (20 ng/mL), and DOX (2 μg/mL). For comparison, control-transduced CD1a⁺ LCs were in parallel stimulated with 2 μg/mL LPS or 500 ng/mL CD40L. IL-6, IL-1β, TNFα, and IL-12 p70 amounts (mean ± SEM of 3 independent experiments) were determined after 48 hours.