Detection of JAK2^V617F expression in transgenic mice. (A) JAK2^V617F copy numbers were determined by real-time PCR assays of genomic DNAs from founder (F0), F1, F3, and F5 generations of A, B, and C line transgenic mice. Data represent ratios of human JAK2^V617F and mouse Jak2 (mean ± SD, n ≥ 6). (B) Real-time PCR assays of JAK2^V617F mRNA expression in the indicated tissues of line A transgenic mice. Data represent relative mRNA levels (mean ± SD, n ≥ 3) normalized to mouse GAPDH in arbitrary units.